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(g) Using the tip of a pulled pipette, cut around the area of undifferentiated cells and cut into smaller equal chunks. Typically a colony of size similar to that in Fig. 2.2 should be cut into 6 10 pieces depending on the rate of regrowth of that particular hES line. (h) When the colony has been cut, lift the free- oating pieces with a pipettor and suitable tip. (i) Transfer the pieces of colony onto new MEF plates. As a guide, equally distribute around 4 6 smaller pieces within a fresh MEF well. (j) Carefully transfer the old and new hES plates back into the incubator. The pieces should be allowed to settle overnight without disturbing the plate, to allow cells to attach and not clump in the center of the plate. (k) The following day, supplement the hES medium in both old and new plates with another 250 L of hES medium to reduce the risk of aspirating unattached hES cells in a full feed. Note. A fresh sterile pulled pipette should be used each time you enter a well to avoid contamination.

The model was organized by:

One occasion when your loop will not require an exit condition is when the loop s purpose is to force a pause (for example, holding the active window in frontmost position) until the user clicks a button. In this case, the button the user clicks may be defined to halt or exit the current script as shown in Figure 13.9.

FIGURE 4.5 Control handles pull the line segment away from the straight line that would normally exist between them. The bottom path is a reversed S shape because the control handles are pulling in opposite directions. Anchor point Control handle

document.writeln(rightNow.getHours() + : + rightNow.getMinutes());

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quality when taken alone; however, in the context of his other drawings and explanations it pointed toward sexual issues related to abuse and inadequacies. His next two renderings placed more pieces within the ensuing puzzle. In Figure 1.20 he drew his experiences of hurt. This rendering is completely symbolic and appears aggressively sexual in nature. He titled the image Love oasis ignorance. All marks with the exception of the linked circles are his experiences of hurt. The raindrop shape symbolized pain, while the word ignorance stood for lack of honor, which in turn stood for a lack of manhood. The linked circles were the love oasis. At the end of the session I asked the patient to place himself within the image; he is found as a small dot just inside the circled area. By Figure 1.21 he was again utilizing shapes within shapes and continued to use sexualized symbols (note the sun). However, on this day he was also verbally fantasizing about how we would set up house and how I would take care of him. With each statement and drawing, it was clear he was regressing into a state of dependency. The disintegration, produced by anxiety, was nding outlet in the safety and comfort of the familiar and a familiar person to take care of his needs. Yet the schizophrenic patient, fearful of closeness, tends to distort feelings and thereby concretize his or her thoughts. As Arieti (1955) has said, physical love is a concrete symbolization of what is really wanted: love and reassurance (p. 477). As a nal remark, in Figure 1.21 the patient is found on the far left with two

That is, the standard error is the square root of the product of the observed probability times one minus the observed probability divided by the sample size. The following SQL query calculates both the standard error and the lower and upper bounds of the 95% confidence interval:

Select the default title (My Great DVD) in the DVD Title box and type the title you want. In the Choose a Theme box, click the theme for the overall look of the DVD.

var theUA = navigator.userAgent.toLowerCase(); isSafari = (theUA.indexOf( safari ) != -1);

Now that you re ready to save your game, plug your PSP into the Xbox USB adapter using the adapter and the USB cable, as shown in Figure 3-17.

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